Biology lab work 4 assistance



UMUC Biology 102/103

Lab 4: Enzymes






·         On your own and extraneously countenance, entire this Lab 4 Answer Sheet electronically and yield it via the Assignments Folder by the duration listed in the Course Schedule (subordinate Syllabus).


·         To spend your laboratory exercises, use the Laboratory Manual located subordinate Course Content. Read the commencement and the directions for each exercise/illustration carefully antecedently completing the exercises/experiments and sympathetic the questions.


·         Save your Lab 4 Answer Sheet in the aftercited format:  LastName_Lab4 (e.g., Smith_Lab4).


·         You should yield your muniment as a Word (.doc or .docx) or Rich Text Format (.rtf) polish for best compatibility.






Pre-Lab Questions




  1. How could you cupel to see if an enzyme was entirely saturated during an illustration?






  1. List three conditions that would substitute the disposition of an enzyme. Be restricted delay your exposition.






  1. Take a seem encircling your stock and test stockhold products that effort by instrument of an enzyme. Name the products, and betoken how you distinguish they effort delay an enzyme.








Experiment 1: Enzymes in Food






Data Tables and Post-Lab Assessment


Table 1: Substance vs. Lugubriousness Presence


Table 1: Substance vs. Lugubriousness Presence


Resulting Color

Presence of Starch?

Positive Control: Ginger Root



Negative Control: Student Must Select



Food Product:



Food Product:









Post-Lab Questions


  1. What were your governs for this illustration? What did they teach? Why was saliva comprised in this illustration?




  1. What is the capacity of amylase? What does amylase do to lugubriousness?




  1. Which of the foods that you cupeled comprehended amylase? Which did not? What illustrational attraction influences your privilege?




  2. Saliva does not comprehend amylase until babies are two months old. How could this pretend an infant’s assimilateive requirements?




  3. There is another assimilateive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to designate what this enzyme is designated. What substrate does it act on? Where in the whole does it beseem activated, and why?




  1. Digestive enzymes in the gut grasp proteases, which assimilate proteins. Why don’t these enzymes assimilate the stomach and slender interior, which are insufficiently tight of protein?






Experiment 2: Effect of Weather on Enzyme Disposition




Data Tables and Post-Lab Assessment


Table 2: Balloon Circumference vs. Temperature




Table 2: Balloon Circumference vs. Temperature


Temperature (°C)

Uninflated Balloon Circumference (cm)

Final Balloon Circumference (cm)

Difference in Balloon Circumference (cm)

1 - (Cold)





2 - (RT)




3 - (Hot)









Post-Lab Questions


  1. What reaction is nature catalyzed in this illustration?


  2. What is the enzyme in this illustration? What is the substrate?


  3. What is the fractions wavering in this illustration? What is the resting wavering?


  4. How does the weather pretend enzyme capacity? Use attraction from your grounds to influence your repartee.


  5. Draw a graph of balloon bisection vs. weather. What is the correspondence?


  6. Is there a disclaiming govern in this illustration? If yes, test the govern. If no, allude-to how you could amend the illustration to grasp a disclaiming govern.


  7. In open, how would an acception in substrate substitute enzyme disposition? Draw a graph to paint this interdependence.


  8. Design an illustration to designate the optimal weather for enzyme capacity, entire delay governs. Where would you experience the enzymes for this illustration? What substrate would you use?