General Biology Lab 1 Assignment 5 What antigens are present on your red blood cells if

General Biology Lab 1Assignment 5Name: ______________________________Part I: Blood Typing: 1. What antigens are present on your red blood cells ifa. Your blood is type B?b. Your blood is type A?c. Your blood is type AB?d. Your blood is type O?2. How might you determine if a donor individual’s blood is compatible with a patient inan emergency situation (e. g. where you don’t have a blood typing kit handy)?3. What would happen to a Type O patient if he receives Type A or Type B blood?4. What would happen to a Type A patient if he receives Type O blood?Part II: ELISA exercise1. The samples that you added to the microplate strip may contain different proteins andmay or may not contain the “disease” antigen. What should happen to the proteins fromthe sample when added to the plastic well if the sample contained the “disease” antigen?2. Why did you need to wash the wells in the microplate stripafter-After addition of sample?-After addition of primary antibody?-After addition of secondary antibody?3. When you added primary antibody to your wells what happened if-your sample contained the antigen?-your sample did not contain the antigen?4. When you added secondary antibody to your wells what happened if-your sample contained the antigen?-your sample did not contain the antigen?5. Why did you run your samples in triplicate in the microplate strip?6. If the sample gave a negative result does this mean that the sample added to the well didnot contain any trace of the “disease” antigen?What reason(s) could you give for a negative result when a sample that was added to thewell actually had the “disease” antigen present?7. According to the table generated in lab, which samples (or sample) were the most likelyto have had the “disease” antigen present before any mixing of samples took place (seetable on the following page)?your tubenumberyourstatus atend(P /N)# MixingTube 1# MixingTube 2# MixingTube 3

General Biology Lab 1 Assignment 5 What antigens are present on your red blood cells if

Question
General Biology Lab 1
Assignment 5

Name: ______________________________

Part I: Blood Typing:
1. What antigens are present on your red blood cells if

a. Your blood is type B?

b. Your blood is type A?

c. Your blood is type AB?

d. Your blood is type O?

2.

How might you determine if a donor individual’s blood is compatible with a patient in
an emergency situation (e.g. where you don’t have a blood typing kit handy)?

3. What would happen to a Type O patient if he receives Type A or Type B blood?

4. What would happen to a Type A patient if he receives Type O blood?

Part II: ELISA exercise

1. The samples that you added to the microplate strip may contain different proteins and
may or may not contain the “disease” antigen. What should happen to the proteins from
the sample when added to the plastic well if the sample contained the “disease” antigen?

2. Why did you need to wash the wells in the microplate stripafter

After addition of sample?

After addition of primary antibody?

After addition of secondary antibody?

3. When you added primary antibody to your wells what happened if

your sample contained the antigen?

your sample did not contain the antigen?

4. When you added secondary antibody to your wells what happened if

your sample contained the antigen?

your sample did not contain the antigen?

5. Why did you run your samples in triplicate in the microplate strip?

6.

If the sample gave a negative result does this mean that the sample added to the well did
not contain any trace of the “disease” antigen?

What reason(s) could you give for a negative result when a sample that was added to the
well actually had the “disease” antigen present?

7. According to the table generated in lab, which samples (or sample) were the most likely
to have had the “disease” antigen present before any mixing of samples took place (see
table on the following page)?

your tube
number

your
status at
end
(P /N)

# Mixing
Tube 1

# Mixing
Tube 2

# Mixing
Tube 3