You use a fluorescence microscope to look at the chromosomes

You use a fluorescence microscope to look at the chromosomes of cells stained with Hoechst33342, a common stain for DNA. The absorption and fluorescence emission spectra for Hoechst33342 are shown in the graph below. Which of the following barrier filters would you select toplace between the sample and the eye piece?

You use a fluorescence microscope to look at the chromosomes

Question
You use a fluorescence microscope to look at the chromosomes of cells stained with Hoechst
33342, a common stain for DNA. The absorption and fluorescence emission spectra for Hoechst
33342 are shown in the graph below. Which of the following barrier filters would you select to
place between the sample and the eye piece?